1M44

Aminoglycoside 2'-N-acetyltransferase from Mycobacterium tuberculosis-APO Structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2001-02-02
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	42.000, 68.500, 120.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 * - 1.600
Rwork	0.174
R-free	0.20100
Structure solution method	Single Anomalous Dispersion
Starting model (for MR)	NONE
RMSD bond length	0.021
RMSD bond angle	2.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	1.700
High resolution limit [Å]	1.600	1.600
Rmerge	0.040 *	0.116 *
Number of reflections	45474 *
<I/σ(I)>	11.6	6.5
Completeness [%]	97.3	86.2 *
Redundancy	3.9 *	2.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.5	298	Ammonium Sulfate, MES, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	MES	100 (mM)	pH5.5
2	1	1	ammonium sulfate	0.8 (M)