1M3D

Structure of Type IV Collagen NC1 Domains

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-11-21
Detector	ADSC QUANTUM 4
Wavelength(s)	0.8856, 0.9195, 0.9197, 0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	80.069, 137.963, 127.131
Unit cell angles	90.00, 90.31, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.171
Rwork	0.169
R-free	0.19700
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.290 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.250	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.034	0.086
Total number of observations	686286 *
Number of reflections	184445
<I/σ(I)>	30.5	13.1
Completeness [%]	97.9	87.8
Redundancy	3.72	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	298	PEG 20K, bicine buffer, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG20000	10 (%(w/v))
3	1	reservoir	Bicine	0.1 (M)	pH9.0