1M3D
Structure of Type IV Collagen NC1 Domains
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.8856, 0.9195, 0.9197, 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.069, 137.963, 127.131 |
| Unit cell angles | 90.00, 90.31, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.171 |
| Rwork | 0.169 |
| R-free | 0.19700 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.290 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.250 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.034 | 0.086 |
| Total number of observations | 686286 * | |
| Number of reflections | 184445 | |
| <I/σ(I)> | 30.5 | 13.1 |
| Completeness [%] | 97.9 | 87.8 |
| Redundancy | 3.72 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | PEG 20K, bicine buffer, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG20000 | 10 (%(w/v)) | |
| 3 | 1 | reservoir | Bicine | 0.1 (M) | pH9.0 |
