1M38
Structure of Inorganic Pyrophosphatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Wavelength(s) | 0.91 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.690, 93.230, 69.840 |
| Unit cell angles | 90.00, 99.74, 90.00 |
Refinement procedure
| Resolution | 17.590 - 1.800 |
| R-factor | 0.1932 |
| Rwork | 0.193 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.561 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | BLANC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 17.600 |
| High resolution limit [Å] | 1.770 |
| Rmerge | 0.037 |
| Number of reflections | 63474 |
| Completeness [%] | 99.9 |
| Redundancy | 4.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | mes-buffer, cobalt chloride, imidodiphosphate, 2-methyl-2,4-pentadiole, pH 6.5, VAPOR DIFFUSION, temperature 298.0K |
