1M2Z

Crystal structure of a dimer complex of the human glucocorticoid receptor ligand-binding domain bound to dexamethasone and a TIF2 coactivator motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2002-08-15
Detector	MACSCIENCE
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	125.843, 125.843, 85.976
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 * - 2.500
R-factor	0.267
Rwork	0.237
R-free	0.26700
Structure solution method	Amore
Starting model (for MR)	GR model built on the PR structure
RMSD bond length	0.007
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNX (2000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.082	0.718
Number of reflections	27095
<I/σ(I)>	35.9	2.5
Completeness [%]	99.4 *
Redundancy	10	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	salts, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6.3 (mg/ml)
2	1	reservoir	HEPES	50 (mM)	pH8.0
3	1	reservoir	ammonium formate	2.0 (M)