1M2K
Sir2 homologue F159A mutant-ADP ribose complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6B |
Synchrotron site | PAL/PLS |
Beamline | 6B |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Detector | MAC Science DIP-2030 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.109, 84.758, 44.742 |
Unit cell angles | 90.00, 109.21, 90.00 |
Refinement procedure
Resolution | 40.000 * - 1.700* |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.22020 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.230 * |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 * |
High resolution limit [Å] | 1.700 * |
Rmerge | 0.031 * |
Total number of observations | 87707 * |
Number of reflections | 26195 * |
Completeness [%] | 94.8 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 | 18 * | PEG 4000, Sodium Citrate, Isopropanol, pH 6.0, EVAPORATION, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mM) | |
2 | 1 | reservoir | sodium citrate | 100 (mM) | pH6.0 |
3 | 1 | reservoir | PEG4000 | 14 (%) | |
4 | 1 | reservoir | isopropanol | 20 (%) |