1M2I
Crystal structure of E44A/E56A mutant of cytochrome b5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1997-12-05 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 70.693, 40.490, 39.199 |
Unit cell angles | 90.00, 111.43, 90.00 |
Refinement procedure
Resolution | 19.900 - 1.800 |
R-factor | 0.193 |
Rwork | 0.193 |
R-free | 0.23200 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1m20 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.900 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.045 | 0.228 |
Number of reflections | 8616 | |
<I/σ(I)> | 18.2 | |
Completeness [%] | 88.9 | 56.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PHOSPHATE BUFFER, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PHOSPHATE BUFFER, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |