Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-12-15 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 114.090, 100.020, 200.090 |
| Unit cell angles | 90.00, 105.79, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| Rwork | 0.227 |
| R-free | 0.25500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | FRAGMENT D FROM STRUCTURE 1FZC |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.084 | 0.311 |
| Total number of observations | 1506048 * | |
| Number of reflections | 121776 | |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 94.8 | 67.8 |
| Redundancy | 3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 * | pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | 0.15 (M) | ||
| 3 | 1 | drop | imidazole | 0.05 (M) | pH7.0 |
| 4 | 1 | reservoir | TMAO | 0.5 (M) | |
| 5 | 1 | reservoir | MES | 0.05 (M) | pH6.0 |
| 6 | 1 | reservoir | 1 (mM) | ||
| 7 | 1 | reservoir | sodium azide | 1 (mM) |
