1M16

Human Acidic Fibroblast Growth Factor. 141 Amino Acid Form with Amino Terminal His Tag and Leu 44 Replaced with Phe (L44F), Leu 73 Replaced with Val (L73V), Val 109 Replaced with Leu (V109L) and Cys 117 Replaced with Val (C117V).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	104
Detector technology	IMAGE PLATE
Collection date	2002-04-24
Detector	Canberra 13-element Germanium detector
Wavelength(s)	0.92
Spacegroup name	C 2 2 21
Unit cell lengths	74.180, 97.013, 108.871
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.700
R-factor	0.221
Rwork	0.220
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jqz
RMSD bond length	0.008
RMSD bond angle	11.100 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.047	0.497
Total number of observations	554851 *
Number of reflections	41034
<I/σ(I)>	32.1	3.3
Completeness [%]	88.3 *	90.5
Redundancy	10.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	sodium formate, ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium phosphate	50 (mM)
2	1	reservoir		100 (mM)
3	1	reservoir	ammonium sulfate	10 (mM)
4	1	reservoir	dithiothreitol	2 (mM)
5	1	reservoir	EDTA	0.5 (mM)	pH7.5
6	1	drop	protein	10-12 (mg/ml)