1M0Z
Crystal Structure of the von Willebrand Factor Binding Domain of Glycoprotein Ib alpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-09-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 121.500, 54.500, 101.800 |
Unit cell angles | 90.00, 103.70, 90.00 |
Refinement procedure
Resolution | 40.000 * - 1.850 |
R-factor | 0.189 |
Rwork | 0.186 |
R-free | 0.21700 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 26.000 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 * | 1.890 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.073 | 0.337 |
Number of reflections | 53895 | |
<I/σ(I)> | 16.3 | 2.9 |
Completeness [%] | 97.8 | 80.8 |
Redundancy | 3.6 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 * | 28 * | ammonium sulfate, lithium sulfate, CAPS, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 301K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris | 25 (mM) | |
2 | 1 | drop | 100 (mM) | pH7.8 | |
3 | 1 | drop | protein | 4 (mg/ml) | |
4 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
5 | 1 | reservoir | lithium sulfate | 0.2 (M) | |
6 | 1 | reservoir | CAPS | 100 (mM) | pH8.2 |