1M0Z
Crystal Structure of the von Willebrand Factor Binding Domain of Glycoprotein Ib alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-09-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.500, 54.500, 101.800 |
| Unit cell angles | 90.00, 103.70, 90.00 |
Refinement procedure
| Resolution | 40.000 * - 1.850 |
| R-factor | 0.189 |
| Rwork | 0.186 |
| R-free | 0.21700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 26.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 * | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.073 | 0.337 |
| Number of reflections | 53895 | |
| <I/σ(I)> | 16.3 | 2.9 |
| Completeness [%] | 97.8 | 80.8 |
| Redundancy | 3.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 * | 28 * | ammonium sulfate, lithium sulfate, CAPS, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 301K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 25 (mM) | |
| 2 | 1 | drop | 100 (mM) | pH7.8 | |
| 3 | 1 | drop | protein | 4 (mg/ml) | |
| 4 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
| 5 | 1 | reservoir | lithium sulfate | 0.2 (M) | |
| 6 | 1 | reservoir | CAPS | 100 (mM) | pH8.2 |
