1M0W
Yeast Glutathione Synthase Bound to gamma-glutamyl-cysteine, AMP-PNP and 2 Magnesium Ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.040 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.661, 52.006, 100.636 |
| Unit cell angles | 82.08, 86.77, 77.49 |
Refinement procedure
| Resolution | 20.000 - 1.820 * |
| R-factor | 0.172 |
| Rwork | 0.172 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m0t |
| RMSD bond length | 0.011 |
| RMSD bond angle | 23.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.066 | 0.400 |
| Total number of observations | 505425 * | |
| Number of reflections | 91335 | |
| <I/σ(I)> | 15.17 | 4.4 |
| Completeness [%] | 97.2 | 95.4 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 22 * | Ammonium sulfate, PEG 400, Tris-HCl pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | gamma-dlutamylcysteine | 3 (mM) | |
| 2 | 1 | drop | AMP-PNP | 3 (mM) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | reservoir | ammonium sulfate | 2.2 (M) | |
| 5 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.0 |
| 6 | 1 | reservoir | PEG400 | 2 (%) | |
| 7 | 1 | reservoir | TCEP | 5 (mM) | |
| 8 | 1 | reservoir | 40 (mM) |
