1LYC
The impact of the physical and chemical enviroment on the molecular structure of Coprinus cinereus peroxidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-09-23 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8346 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 77.289, 115.055, 36.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.890 - 1.570 |
R-factor | 0.223 |
Rwork | 0.223 |
R-free | 0.26500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | future PDB deposition |
RMSD bond length | 0.019 |
RMSD bond angle | 22.000 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.600 |
High resolution limit [Å] | 1.570 | 1.570 |
Rmerge | 0.073 * | 0.223 |
Total number of observations | 155261 * | |
Number of reflections | 52771 * | |
Completeness [%] | 88.2 * | 96 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 4.25 | 293 | 1.5M-1.6M NH4H2PO, pH 4.0-4.5, VAPOR DIFFUSION, HANGING DROP, pH 4.25, temperature 293K |
1 | Vapor diffusion * | 4.25 | 293 | 1.5M-1.6M NH4H2PO, pH 4.0-4.5, VAPOR DIFFUSION, HANGING DROP, pH 4.25, temperature 293K |
1 | Vapor diffusion * | 4.25 | 293 | 1.5M-1.6M NH4H2PO, pH 4.0-4.5, VAPOR DIFFUSION, HANGING DROP, pH 4.25, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 1.5-1.6 (M) | ||
2 | 1 | drop | protein | 17-21 (mg/ml) |