1LY1
Structure and Mechanism of T4 Polynucleotide Kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9790 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 65.300, 91.800, 67.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.000 |
| Rwork | 0.210 |
| R-free | 0.23100 |
| Structure solution method | SIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.060 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.051 | 0.223 |
| Total number of observations | 36111 * | |
| Number of reflections | 12892 | |
| <I/σ(I)> | 15.3 | 4.3 |
| Completeness [%] | 89.2 | 77.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 22 * | 0.1M NaOAc 4.6, 5mM DTT, 16% PEG2000MME, 0.4M AMSO4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | pH7.5 |
| 3 | 1 | drop | 0.2 (M) | ||
| 4 | 1 | drop | imidazole | 0.3 (M) | |
| 5 | 1 | drop | glycerol | 10 (%) | |
| 6 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.6 |
| 7 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 8 | 1 | reservoir | PEG2000 MME | 16 (%) | |
| 9 | 1 | reservoir | ammonium sulfate | 0.4 (M) |
