1LXZ
Structure of thaumatin crystallized in the presence of glycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-12-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9474 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.801, 57.801, 149.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.250 |
| R-factor | 0.179 |
| Rwork | 0.178 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1thw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.581 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.290 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Number of reflections | 69080 | |
| Completeness [%] | 96.8 | 96.9 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | glycerol, tartrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
