1LXN
X-RAY STRUCTURE OF MTH1187 NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET TT272
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-02-27 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9791 |
Spacegroup name | P 41 3 2 |
Unit cell lengths | 175.365, 175.365, 175.365 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.300 |
Rwork | 0.216 |
R-free | 0.24500 |
Structure solution method | MIR |
RMSD bond length | 0.005 |
RMSD bond angle | 23.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.058 * | |
Total number of observations | 425012 * | |
Number of reflections | 40380 | |
Completeness [%] | 100.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8.5 * | 4 * | |
1 | Vapor diffusion * | 8.5 * | 4 * | |
1 | Vapor diffusion * | 8.5 * | 4 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | HEPES | 10 (mM) | |
2 | 1 | drop | 500 (mM) | ||
3 | 1 | drop | protein | 20 (mg/ml) | |
4 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
5 | 1 | reservoir | ammonium sulfate | 2 (M) | |
6 | 1 | reservoir | glycerol | 14 (%) |