1LXJ

X-RAY STRUCTURE OF YBL001c NORTHEAST STRUCTURAL GENOMICS (NESG) CONSORTIUM TARGET YTYst72

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-04
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97895
Spacegroup name	I 41 2 2
Unit cell lengths	67.380, 67.380, 133.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 1.800
Rwork	0.211
R-free	0.24300
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	23.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.096 *
Total number of observations	141589 *
Number of reflections	12726
Completeness [%]	86.8	85.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.5 *	4 *	Ammonium Sulfate, 2-METHYL-2,4-PENTANEDIOL, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HEPES	10 (mM)
2	1	drop		500 (mM)
3	1	drop	protein	20 (mg/ml)
4	1	reservoir	Tris	0.1 (M)	pH8.5
5	1	reservoir	ammonium sulfate	2 (M)
6	1	reservoir	MPD	12 (%)