1LX7
Structure of E. coli uridine phosphorylase at 2.0A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-11-15 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9794 |
Spacegroup name | H 3 |
Unit cell lengths | 151.375, 151.375, 48.151 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 * - 2.000 |
R-factor | 0.182 * |
Rwork | 0.182 |
R-free | 0.21500 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.860 |
High resolution limit [Å] | 2.000 * | 2.000 * |
Rmerge | 0.038 * | 0.080 * |
Total number of observations | 747216 * | |
Number of reflections | 49742 * | |
Completeness [%] | 89.2 * | 78.6 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 291 | 0.1M Mes, 10% PEG 4K, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | pH8. |
3 | 1 | drop | 50 (mM) | ||
4 | 1 | drop | dithiothreitol | 1 (mM) | |
5 | 1 | reservoir | PEG4000 | 10 (%) | |
6 | 1 | reservoir | MES | 0.1 (M) | pH6.5 |
7 | 1 | reservoir | glycerol | 5 (%) |