1LVF
syntaxin 6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-01-01 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.600, 54.380, 94.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.550 - 2.100 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.20600 * |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.873 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.190 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.099 * | 0.345 * |
| Number of reflections | 11792 | |
| Completeness [%] | 99.6 * | 96.7 |
| Redundancy | 4.1 * | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.4 | 21 * | PEG 8000, sodium acetate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 150 (mM) | ||
| 2 | 1 | drop | beta-mercaptoethanol | 5 (mM) | |
| 3 | 1 | drop | HEPES | 10 (mM) | pH7.4 |
| 4 | 1 | reservoir | PEG8000 | 30 (%(v/v)) | |
| 5 | 1 | reservoir | sodium acetate | 400 (mM) | |
| 6 | 1 | reservoir | beta-mercaptoethanol | 10 (mM) | |
| 7 | 1 | reservoir | HEPES | 10 (mM) | pH7.4 |
