1LV8

Crystal structure of calf spleen purine nucleoside phosphorylase in a new space group with full trimer in the asymmetric unit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-09-01
Detector	MARRESEARCH
Wavelength(s)	0.9101
Spacegroup name	P 21 21 21
Unit cell lengths	79.140, 134.235, 177.148
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.300
R-factor	0.189
Rwork	0.189
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fxu
RMSD bond length	0.013
RMSD bond angle	1.780 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	2.440
High resolution limit [Å]	2.300	2.300
Rmerge	0.055	0.377 *
Number of reflections	84155 *
Completeness [%]	99.7	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.25	18 *	HEPES, PEG400, CaCl2, pH 7.25, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	10 (mg/ml)
2	1	reservoir		0.2 (M)
3	1	reservoir	PEG400	19-31 (%)
4	1	reservoir	HEPES	0.1 (M)	pH7.25