1LV8
Crystal structure of calf spleen purine nucleoside phosphorylase in a new space group with full trimer in the asymmetric unit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-09-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9101 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.140, 134.235, 177.148 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.300 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fxu |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.780 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.055 | 0.377 * |
| Number of reflections | 84155 * | |
| Completeness [%] | 99.7 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.25 | 18 * | HEPES, PEG400, CaCl2, pH 7.25, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 10 (mg/ml) | |
| 2 | 1 | reservoir | 0.2 (M) | ||
| 3 | 1 | reservoir | PEG400 | 19-31 (%) | |
| 4 | 1 | reservoir | HEPES | 0.1 (M) | pH7.25 |
