1LUJ
Crystal Structure of the Beta-catenin/ICAT Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Detector technology | CCD |
| Collection date | 2002-02-16 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9196 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 95.174, 98.504, 86.486 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.740 - 2.510 * |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 18.800 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.510 * | 2.500 |
| Rmerge | 0.119 * | 0.650 * |
| Total number of observations | 163681 * | |
| Number of reflections | 23804 | |
| <I/σ(I)> | 15.6 | 2.2 |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG800, magnesium chloride, Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 4 (%(w/v)) | |
| 3 | 1 | reservoir | 20 (mM) | ||
| 4 | 1 | reservoir | Tris | 50 (mM) | pH8.0 |
| 5 | 1 | reservoir | dithiothreitol | 5 (mM) |
