1LUF
Crystal Structure of the MuSK Tyrosine Kinase: Insights into Receptor Autoregulation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-04-29 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.979 |
Spacegroup name | I 4 |
Unit cell lengths | 146.939, 146.939, 39.017 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 * - 2.050 |
R-factor | 0.231 |
Rwork | 0.231 |
R-free | 0.25200 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 1IRK |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.300 * |
Data reduction software | MADNESS |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 * | 2.100 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.056 * | 0.319 * |
Total number of observations | 216370 * | |
Number of reflections | 28438 * | |
Completeness [%] | 99.7 | 99.1 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 20 * | ammonium sulfate, HEPES, glycerol, TCEP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 0.75 (M) | |
3 | 1 | reservoir | HEPES | 0.1 (M) | pH7.0 |
4 | 1 | reservoir | glycerol | 2 (%) | |
5 | 1 | reservoir | TCEP | 1.3 (mM) | |
6 | 1 | reservoir | sodium thiocyanate | 0.1 (M) |