1LTQ
CRYSTAL STRUCTURE OF T4 POLYNUCLEOTIDE KINASE
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97873 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 78.585, 93.684, 124.165 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.330 |
| R-factor | 0.238 |
| Rwork | 0.236 |
| R-free | 0.26100 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.400 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.073 | 0.288 |
| Total number of observations | 191084 * | |
| Number of reflections | 38288 | |
| <I/σ(I)> | 12.8 | 5.7 |
| Completeness [%] | 99.1 | 97.7 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 298 | PEG 4000, potassium chloride, MES, Tris, ATP, DTT, EDTA, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 10 (mM) | pH7.4 |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | EDTA | 0.1 (mM) | |
| 5 | 1 | drop | ATP | 0.1 (mM) | |
| 6 | 1 | drop | protein | 1 (mg/ml) | |
| 7 | 1 | reservoir | PEG4000 | 5-10 (%) | |
| 8 | 1 | reservoir | MES | 0-5 (mM) | pH6.5 |
