1LTI
HEAT-LABILE ENTEROTOXIN (LT-I) COMPLEX WITH T-ANTIGEN
Experimental procedure
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-12-10 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 119.700, 100.500, 64.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.130 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.27400 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 23.000 * |
| Data reduction software | RAXIS (SOFTWARE) |
| Data scaling software | R-AXIS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.000 | 2.250 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.054 | 0.160 |
| Number of reflections | 38264 | |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 86.2 | 73.5 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | three-layer capillary * | 7.5 | LIQUID-LIQUID DIFFUSION CAPILLARY CONTAINS: 0.1M TRIS PH 7.5, 0.1M NACL, 0.001M EDTA, 0.02% SODIUM AZIDE, 4.5 MG/ML LT-I, 0.025M LACTOSE AND 5% PEG 6000. CRYSTAL WAS SOAKED FOR 1 DAY IN LACTOSE FREE MOTHER LIQUOR CONTAINING 0.1M D-GAL-BETA1,3GALNAC BEFORE DATA COLLECTION., liquid - liquid diffusion |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | LT | 9 (mg/ml) | |
| 2 | 1 | 1 | Tris | 100 (mM) | |
| 3 | 1 | 1 | 200 (mM) | ||
| 4 | 1 | 1 | EDTA | 1 (mM) | |
| 5 | 1 | 1 | 0.02 (%) | ||
| 6 | 1 | 2 | lactose | 166 (mM) | |
| 7 | 1 | 2 | Tris | 100 (mM) | |
| 8 | 1 | 3 | PEG6000 | 14 (%) | |
| 9 | 1 | 3 | Tris | 100 (mM) |
