1LTH

T AND R STATES IN THE CRYSTALS OF BACTERIAL L-LACTATE DEHYDROGENASE REVEAL THE MECHANISM FOR ALLOSTERIC CONTROL

Experimental procedure

Spacegroup name	F 2 2 2
Unit cell lengths	148.400, 295.900, 71.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.188
Rwork	0.188
RMSD bond length	0.013
RMSD bond angle	1.631
Phasing software	X-PLOR (2.1)
Refinement software	X-PLOR (2.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	2.500
Rmerge	0.055 *
Total number of observations	115542 *
Number of reflections	24341
Completeness [%]	97.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		10 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	NADH	2.5 (mM)
2	1	reservoir	oxamate	2.5 (mM)
3	1	reservoir	FBP	0.5 (mM)
4	1	reservoir	PEG6000	20 (%)