1LT9
Crystal Structure of Recombinant Human Fibrinogen Fragment D
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-08-27 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.727, 94.511, 227.111 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 17.980 - 2.800 |
Rwork | 0.224 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.270 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.110 * | 0.540 * |
Total number of observations | 217604 * | |
Number of reflections | 47742 | |
<I/σ(I)> | 14 | 3.1 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 4 * | 9% PEG 3350, 70mM Calcium Chloride, 2mM Sodium Azide, 50mM Tris pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12 (mg/ml) | |
3 | 1 | reservoir | Tris | 50 (mM) | pH8.5 |
4 | 1 | reservoir | 2 (mM) | ||
5 | 1 | reservoir | 70 (mM) | ||
6 | 1 | reservoir | PEG3350 | 9 (%(w/v)) |