1LSO
Crystal Structure of the S137A mutant of L-3-Hydroxyacyl-CoA Dehydrogenase in Complex with NAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-07-02 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.361, 86.452, 167.687 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.190 - 2.600 |
| R-factor | 0.221 |
| Rwork | 0.220 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3had |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | Crystal (Clear / d*trek) |
| Data scaling software | CrystalClear (D*TREK (MSC/RIGAKU)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.190 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.071 | 0.272 |
| Number of reflections | 23099 | |
| <I/σ(I)> | 14.8 | 6.1 |
| Completeness [%] | 98.9 | 99.6 |
| Redundancy | 3.83 | 3.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 50 mM N-[2-ACETAMIDO]-2-IMINODIACETIC ACID WITHIN THE PRECIPITANT RANGE OF 14% TO 19% POLYETHYLENE GLYCOL 4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
