1LSL

Crystal Structure of the Thrombospondin-1 Type 1 Repeats

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2000-12-17
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00803, 1.00808, 1.0596
Spacegroup name	C 1 2 1
Unit cell lengths	67.630, 84.410, 37.170
Unit cell angles	90.00, 108.67, 90.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.235
Rwork	0.238
R-free	0.28200
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	27.854 *
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MLPHARE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.075	0.469
Total number of observations	162978 *
Number of reflections	15485 *
<I/σ(I)>	14.6	3.8
Completeness [%]	99.8	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	0.5 M sodium potassium tartrate and 0.1 M sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	0.5 M sodium potassium tartrate and 0.1 M sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium potassium tartrate	0.5 (M)
2	1	reservoir	sodium acetate	0.1 (M)	pH5.5