1LRI

BETA-CRYPTOGEIN-CHOLESTEROL COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	2001-12-17
Detector	MARRESEARCH
Wavelength(s)	0.964
Spacegroup name	C 2 2 21
Unit cell lengths	30.964, 94.800, 65.296
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 * - 1.450
R-factor	0.127
Rwork	0.127
R-free	0.16800 *
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1bxm
RMSD bond length	0.012
RMSD bond angle	0.030 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000 *	1.480
High resolution limit [Å]	1.450	1.448
Rmerge	0.390	0.213 *
Total number of observations	64233 *
Number of reflections	17432
<I/σ(I)>	16.5	4.1
Completeness [%]	99.1	96 *
Redundancy	3.9	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	290	sodium chloride, cholesterol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		4.5 (M)