1LR6

Crystal structure of V45Y mutant of cytochrome b5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1999-11-10
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	69.404, 40.441, 39.196
Unit cell angles	90.00, 111.70, 90.00

Refinement procedure

Resolution	22.820 - 1.900
R-factor	0.191
Rwork	0.191
R-free	0.23500 *
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1ehb
RMSD bond length	0.008
RMSD bond angle	1.165 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.820	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.062	0.194
Number of reflections	7563
<I/σ(I)>		6.1
Completeness [%]	93.5	76.3
Redundancy	3.1	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	PHOSPHATE BUFFER, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	phosphate	3.1 (M)	pH7.5