1LQM

ESCHERICHIA COLI URACIL-DNA GLYCOSYLASE COMPLEX WITH URACIL-DNA GLYCOSYLASE INHIBITOR PROTEIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	98.757, 158.875, 91.222
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 3.200
R-factor	0.188
Rwork	0.188
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uug
RMSD bond length	0.011
RMSD bond angle	26.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	3.310
High resolution limit [Å]	3.200	3.200
Rmerge	0.167 *	0.398 *
Total number of observations	94765 *
Number of reflections	21106
<I/σ(I)>	9.2	1.4
Completeness [%]	87.6	79.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	281 *	20mM imidazole-maleate, 10% PEG 4000, pH 7.6, VAPOR DIFFUSION, HANGING DROP at 293K, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	imidazole malate	20 (mM)	pH7.6
3	1	reservoir	PEG4000	10 (%(w/v))