1LQL

Crystal structure of OsmC like protein from Mycoplasma pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97919
Spacegroup name	P 21 21 21
Unit cell lengths	66.360, 95.600, 308.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.850
R-factor	0.219
Rwork	0.217
R-free	0.27600
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	24.500 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.920
High resolution limit [Å]	2.850	2.850
Rmerge	0.091 *	0.391 *
Number of reflections	90144	6014 *
<I/σ(I)>	23.7	4.1
Completeness [%]	99.9	99.9
Redundancy	5.0 *	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	PEG 3350, sodium iodide, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12.9 (mg/ml)
2	1	drop	Tris	20 (mM)	pH7.5
3	1	drop	EDTA	1 (mM)
4	1	drop	dithiothreitol	1 (mM)
5	1	drop		300 (mM)
6	1	reservoir	sodium iodide	0.2 (M)	pH6.9
7	1	reservoir	PEG3350	20 (%)