1LQK

High Resolution Structure of Fosfomycin Resistance Protein A (FosA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	113
Detector technology	CCD
Collection date	2002-02-16
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0062
Spacegroup name	P 21 21 21
Unit cell lengths	54.788, 66.967, 77.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.350
R-factor	0.138 *
Rwork	0.137
R-free	0.17660
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	2.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.400
High resolution limit [Å]	1.350	1.350
Rmerge	0.030	0.220 *
Number of reflections	55325
<I/σ(I)>	38.6	5.3
Completeness [%]	92.6	58.6
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	WELL CONTAINED 40% PENTAERYTHRITOL PROPOXYLATE 629, 0.08M K2HPO4. DROP CONTAINED 0.002 M MNCL2, 0.002M FOSFOMYCIN, 10 MG/ML FOSA, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K