1LQJ

ESCHERICHIA COLI URACIL-DNA GLYCOSYLASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 32
Unit cell lengths	125.210, 125.210, 90.047
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 3.350
R-factor	0.241
Rwork	0.241
R-free	0.32600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uug
RMSD bond length	0.014
RMSD bond angle	27.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.470
High resolution limit [Å]	3.350	3.350
Rmerge	0.154 *	0.369 *
Total number of observations	38941 *
Number of reflections	20394
<I/σ(I)>	6.9	1.4
Completeness [%]	90.2	94.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	20mM Tris-HCl, 100mM NaCl, 15% PEG 8000, pH 7.4, VAPOR DIFFUSION, HANGING DROP at 293K, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)	pH7.4
3	1	drop		100 (mM)
4	1	reservoir	PEG8000	15 (%(w/v))