1LPL

Structural Genomics of Caenorhabditis elegans: CAP-Gly domain of F53F4.3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-10
Detector	MARRESEARCH
Wavelength(s)	1.74
Spacegroup name	P 61 2 2
Unit cell lengths	64.156, 64.156, 101.946
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 * - 1.770
R-factor	0.225 *
Rwork	0.220
R-free	0.29400 *
Structure solution method	SAS
RMSD bond length	0.021 *
RMSD bond angle	1.700 *
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	ISAS (V. 2001)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.880
High resolution limit [Å]	1.770	1.770
Rmerge	0.043 *	0.369 *
Number of reflections	12651 *
Completeness [%]	99.2 *	95 *
Redundancy	9.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.4 *	298	Reservoir solution contained 1.8 M ammonium sulphate and 0.1 M MES buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.8 (M)
2	1	reservoir	MES	0.1 (M)	pH6.5
3	1	drop	protein	10 (mg/ml)
4	1	drop	HEPES	20 (mM)	pH7.4