1LPJ

Human cRBP IV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2002-02-14
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9393
Spacegroup name	C 1 2 1
Unit cell lengths	34.053, 57.186, 67.939
Unit cell angles	90.00, 104.03, 90.00

Refinement procedure

Resolution	24.440 - 2.000
Rwork	0.230
R-free	0.27600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1crb
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.600 *	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.124	0.382
Total number of observations	19128 *
Number of reflections	7611
<I/σ(I)>	3.8	1.3
Completeness [%]	89.6	91.4 *
Redundancy	2.7	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	18 *	10% PEG 4000, 10% isopropanol, 100 mM sodium citrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	10 (%(w/v))
2	1	reservoir	2-propanol	10 (%(v/v))
3	1	reservoir	sodium citrate	50 (mM)	pH5.6
4	1	drop	protein	8 (mg/ml)