1LOL

Crystal structure of orotidine monophosphate decarboxylase complex with XMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2001-09-12
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	57.570, 55.482, 66.129
Unit cell angles	90.00, 94.28, 90.00

Refinement procedure

Resolution	30.000 * - 1.900
R-factor	0.193
Rwork	0.193
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	22.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.085 *	0.312 *
Total number of observations	376089 *
Number of reflections	32685
Completeness [%]	99.0	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	trisodium citrate, (+/-)1,3-butanediol, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	trisodium citrate	1.2 (M)	pH6.5-8.5
2	1	reservoir	(+-)-1,3-butanediol	10 (%(v/v))