1LN0
Structure of the Catalytic Domain of Homing Endonuclease I-TevI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-08-01 |
| Detector | BRANDEIS - B1.2 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 123.870, 56.580, 35.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.25500 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.034 | 0.124 |
| Number of reflections | 16242 * | |
| <I/σ(I)> | 33.4 | 8.3 |
| Completeness [%] | 92.2 | 61.6 |
| Redundancy | 3.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | 22% PEG8000, 0.1 M MES, 5% ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | pH7.5 |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | 0.01 (%(w/v)) | ||
| 6 | 1 | reservoir | PEG8000 | 22 (%(w/v)) | |
| 7 | 1 | reservoir | MES | 100 (mM) | pH6.5 |
| 8 | 1 | reservoir | ammonium sulfate | 5 (%(w/v)) |
