1LM7
Structures of two intermediate filament-binding fragments of desmoplakin reveal a unique repeat motif structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-05-07 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.293, 99.773, 51.687 |
Unit cell angles | 90.00, 97.58, 90.00 |
Refinement procedure
Resolution | 100.000 * - 3.000 |
R-factor | 0.249 |
Rwork | 0.249 |
R-free | 0.29600 |
Structure solution method | SAD |
RMSD bond length | 0.095 * |
RMSD bond angle | 1.460 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 * | 3.000 |
Rmerge | 0.087 * | 0.230 * |
Total number of observations | 208172 * | |
Number of reflections | 9183 * | |
Completeness [%] | 93.8 | 84.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 20 * | PEG1500, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 25 (mg/ml) | |
2 | 1 | reservoir | MES | 100 (mM) | pH6.0 |
3 | 1 | reservoir | PEG1500 | 35 (%(w/v)) |