1LLS
CRYSTAL STRUCTURE OF UNLIGANDED MALTOSE BINDING PROTEIN WITH XENON
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-31 |
| Detector | ADSC |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.207, 44.026, 57.649 |
| Unit cell angles | 100.75, 101.36, 102.62 |
Refinement procedure
| Resolution | 17.070 - 1.800 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.22600 |
| Structure solution method | Isomorphous with Structure in Database |
| Starting model (for MR) | 1omp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 22.600 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.070 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.029 * | |
| Total number of observations | 55908 * | |
| Number of reflections | 29855 | |
| <I/σ(I)> | 19.9 | 10.4 |
| Completeness [%] | 92.3 | 76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 298 | 10 mM Sodium Citrate, 23% PEG 8000, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 16 (mg/ml) | |
| 2 | 1 | reservoir | sodium citrate | 10 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 23 (%(w/v)) | pH6.6 |
