1LLO

HEVAMINE A (A PLANT ENDOCHITINASE/LYSOZYME) COMPLEXED WITH ALLOSAMIDIN

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1995-07-18
Detector	MARRESEARCH
Wavelength(s)	0.92
Spacegroup name	P 21 21 21
Unit cell lengths	52.330, 57.990, 82.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.850
R-factor	0.146
R-free	0.20000
RMSD bond length	0.014 *
RMSD bond angle	1.700 *
Data reduction software	DENZO
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.820 *	1.820 *
Rmerge	0.039	0.149 *
Total number of observations	91378 *
Number of reflections	22218 *
Completeness [%]	98.7 *	0.732 *
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4	277 *	pH 4.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	BES	20 (mM)
3	1	reservoir	sodium chloride	1.7-3.4 (M)