1LKV

Crystal Structure of the Middle and C-terminal Domains of the Flagellar Rotor Protein FliG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-05-16
Detector	ADSC QUANTUM 4
Wavelength(s)	0.99981
Spacegroup name	P 64 2 2
Unit cell lengths	113.943, 113.943, 128.826
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.000 * - 2.800
R-factor	0.2574
Rwork	0.255
R-free	0.28000 *
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.972 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.035 *	2.900
High resolution limit [Å]	2.800 *	2.800
Rmerge	0.084 *	0.235 *
Total number of observations	179161 *
Number of reflections	12745 *
Completeness [%]	96.0 *	90.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8 *	298	Isopropanol, CaCl2, sodium acetate, pluronic F-68, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	8 *	298	Isopropanol, CaCl2, sodium acetate, pluronic F-68, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	8 *	298	Isopropanol, CaCl2, sodium acetate, pluronic F-68, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	10 (mM)	pH8.0
2	1	drop		10 (mM)
3	1	drop	protein	10 (mg/ml)
4	1	reservoir	isopropanol	8-10 (%(v/v))
5	1	reservoir		200-400 (mM)
6	1	reservoir	sodium acetate	100 (mM)	pH4.4-5.0
7	1	reservoir	pluronic F-68	1 (%)