1LK3
ENGINEERED HUMAN INTERLEUKIN-10 MONOMER COMPLEXED TO 9D7 FAB FRAGMENT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-11-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.554, 75.616, 111.975 |
| Unit cell angles | 90.00, 96.85, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.910 |
| R-factor | 0.1872 |
| Rwork | 0.187 |
| R-free | 0.24010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.590 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.049 * | 0.340 * |
| Total number of observations | 357669 * | |
| Number of reflections | 96708 | |
| <I/σ(I)> | 10.5 | 1.9 |
| Completeness [%] | 91.0 | 72.6 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 4.6 | 4 * | peg 4000, sodium citrate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | PEG4000 | 8 (%) | |
| 3 | 1 | drop | sodium acetate | 0.1 (M) | pH4.6 |
| 4 | 1 | reservoir | PEG4000 | 12-14 (%) | |
| 5 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
