1LJX
THE STRUCTURE OF D(TPGPCPGPCPA)2 AT 293K: COMPARISON OF THE EFFECT OF SEQUENCE AND TEMPERATURE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-04-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 21.182, 28.363, 44.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.000 - 1.640 |
| R-factor | 0.212 * |
| Rwork | 0.212 |
| R-free | 0.26800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1DCG with its terminal bases modified |
| Data reduction software | AUTOMAR |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.000 |
| High resolution limit [Å] | 1.600 * |
| Rmerge | 0.040 * |
| Number of reflections | 3468 |
| Completeness [%] | 97.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | cacodylate, MgCl2, cobalt hexaamine chloride, MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | cacodylate | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | cobalt hexaamine chloride | ||
| 4 | 1 | 1 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 10 (mM) | pH7.0 |
| 2 | 1 | drop | cobalt hexammine chloride | 150 (mM) | |
| 3 | 1 | drop | 25 (mM) | ||
| 4 | 1 | reservoir | methyl pentanediol | 35 (%) |
