1LJL
Wild Type pI258 S. aureus arsenate reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2002-01-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.450, 32.770, 100.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 * - 2.000* |
| R-factor | 0.23 |
| Rwork | 0.214 |
| R-free | 0.25400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jf8 |
| RMSD bond length | 0.011 * |
| RMSD bond angle | 1.550 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 * | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 * | 0.451 * |
| Total number of observations | 203478 * | |
| Number of reflections | 7503 | |
| <I/σ(I)> | 22.6 | 3.05 |
| Completeness [%] | 96.5 * | 74.8 |
| Redundancy | 7 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8 | 20 * | PEG4000, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 393K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.0 |
| 4 | 1 | reservoir | ammonium bicarbonate | 50 (mM) | |
| 5 | 1 | reservoir | PEG4000 | 42.5 (%(w/v)) |
