1LIK

STRUCTURE OF T. GONDII ADENOSINE KINASE BOUND TO ADENOSINE

Replaces:  1DH0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1998-01-27
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 21 21 2
Unit cell lengths	168.200, 47.010, 44.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.500 *
R-factor	0.13 *
Rwork	0.150
R-free	0.20900 *
RMSD bond length	0.010 *
RMSD bond angle	2.150 *
Phasing software	EPMR
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.680
High resolution limit [Å]	2.500 *	2.550
Rmerge	0.037	0.109
Total number of observations	30369 *
Number of reflections	17172 *
<I/σ(I)>	10
Completeness [%]	97.0 *	81 *
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5		pH 6.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	adenosine	5 (mM)
3	1	reservoir		1.1 (M)
4	1	reservoir		10 (mM)
5	1	reservoir	sodium cacodylate	50 (mM)	pH6.5