1LI5
Crystal Structure of Cysteinyl-tRNA Synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-01-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 119.160, 119.160, 144.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.890 - 2.300 |
| R-factor | 0.245 * |
| Rwork | 0.245 |
| R-free | 0.28300 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.010 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.069 | 0.307 |
| Number of reflections | 45949 | |
| <I/σ(I)> | 16.4 | 4.6 |
| Completeness [%] | 98.8 | 99.8 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 17 * | 16% PEG 8000, 200 MM magnesium acetate, 1MM DTT, cacodylate, 5MM ATP, 10MM cysteine. 100 MM BUFFER, pH 6.5, VAPOR DIFFUSION, HANGING DROP at 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 10 | 1 | reservoir | magnesium acetate | 200 (mM) | |
| 11 | 1 | reservoir | t-butanol | 2 (%) | |
| 2 | 1 | drop | HEPES | 10 (mM) | pH7.4 |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | 5 (mM) | ||
| 6 | 1 | drop | ATP | 5 (mM) | |
| 7 | 1 | drop | cysteine | 10 (mM) | |
| 8 | 1 | reservoir | PEG8000 | 15-17 (%) | |
| 9 | 1 | reservoir | sodium cacodylate | 100 (mM) | pH6.5 |
