1LHZ

Structure of a Human Bence-Jones Dimer Crystallized in U.S. Space Shuttle Mission STS-95: 293K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1998-11-19
Detector	MARRESEARCH
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	48.940, 85.170, 114.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.300
Rwork	0.173
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jvk
RMSD bond length	0.130
RMSD bond angle	1.780
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.077	0.161 *
Total number of observations	92994 *
Number of reflections	21254 *	1943 *
<I/σ(I)>	16.1	7.6
Completeness [%]	98.3	91.7
Redundancy	4.4	3.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *		279	Alvarado, U.R., (2001) J. Crystal Growth, 223, 407. *