1LGB

INTERACTION OF A LEGUME LECTIN WITH THE N2 FRAGMENT OF HUMAN LACTOTRANSFERRIN OR WITH THE ISOLATED BIANTENNARY GLYCOPEPTIDE: ROLE OF THE FUCOSE MOIETY

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	63.500, 63.500, 251.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 3.300
R-factor	0.21
Rwork	0.210
RMSD bond length	0.009
RMSD bond angle	1.700 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	3.300 *
Rmerge	0.160 *
Total number of observations	31028 *
Number of reflections	7766 *
Completeness [%]	96.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *			taken from Bourne, Y. et al. (1992). J. Mol. Biol., 227, 938-941. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	N2 fragment	8.5 (mg/ml)
3	1	reservoir	PEG6000	5-9 (%)
4	1	reservoir	HEPES	0.1 (M)
5	1	reservoir		0.1 (M)