1LG7

Crystal structure of Vesicular Stomatitis Virus Matrix Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	200
Detector technology	IMAGE PLATE
Collection date	1999-09-17
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.00
Spacegroup name	P 4 21 2
Unit cell lengths	80.690, 80.690, 51.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.960
R-factor	0.206 *
Rwork	0.208
R-free	0.24100
Structure solution method	SIRAS
RMSD bond length	0.005 *
RMSD bond angle	1.470 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.010
High resolution limit [Å]	1.960	1.960
Rmerge	0.069 *	0.410 *
Total number of observations	209741 *
Number of reflections	12771 *
<I/σ(I)>	30	3.5
Completeness [%]	99.6	95.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	Gaudier, M., (2001) Virology, 288, 308. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	20 (mM)	pH8.5
2	1	drop		100 (mM)
3	1	drop	protein	5 (mg/ml)
4	1	reservoir	PEG2000 MME	24 (%)
5	1	reservoir	sodium acetate	0.2 (M)
6	1	reservoir		0.1 (M)
7	1	reservoir	Tris-HCl	0.1 (M)	pH8.5