1LG7
Crystal structure of Vesicular Stomatitis Virus Matrix Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-09-17 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 80.690, 80.690, 51.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.960 |
| R-factor | 0.206 * |
| Rwork | 0.208 |
| R-free | 0.24100 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.005 * |
| RMSD bond angle | 1.470 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.010 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.069 * | 0.410 * |
| Total number of observations | 209741 * | |
| Number of reflections | 12771 * | |
| <I/σ(I)> | 30 | 3.5 |
| Completeness [%] | 99.6 | 95.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | Gaudier, M., (2001) Virology, 288, 308. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 20 (mM) | pH8.5 |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | protein | 5 (mg/ml) | |
| 4 | 1 | reservoir | PEG2000 MME | 24 (%) | |
| 5 | 1 | reservoir | sodium acetate | 0.2 (M) | |
| 6 | 1 | reservoir | 0.1 (M) | ||
| 7 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |
