1LFO

LIVER FATTY ACID BINDING PROTEIN-OLEATE COMPLEX

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	287
Detector technology	AREA DETECTOR
Collection date	1991-08-26
Detector	SIEMENS
Spacegroup name	P 32 2 1
Unit cell lengths	83.890, 83.890, 44.650
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.202
Rwork	0.202
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SUPERIMPOSED POLYALANINE COMPOSITE STRUCTURE OF 1ADL 1cbr 1crb 1opb 1ifc 1hmr
RMSD bond length	0.008
RMSD bond angle	27.700 *
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.800	2.300
High resolution limit [Å]	2.100	2.100
Rmerge	0.100 *	0.535 *
Number of reflections	8640	940 *
<I/σ(I)>	10.7	0.89
Completeness [%]	79.3	33.2
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	4 *	HANGING DROP VAPOR DIFFUSION EXPERIMENT 1 ML WELL: 3 M AMMONIUM SULFATE, 200 MM LISO4, 100 MM CITRATE, AT A PH OF 5.6 STOCK: 13 MG/ML LFABP-OLEATE COMPLEX 10 MICROLITER DROP: 1:1 MIXTURE OF STOCK AND WELL, vapor diffusion - hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	3 (M)
2	1	reservoir		200 (mM)
3	1	reservoir	citrate	100 (mM)
4	1	drop	ammonium sulfate	1.5 (M)
5	1	drop		100 (mM)
6	1	drop	citrate	50 (mM)
7	1	drop	protein	6.5 (mg/ml)