1LFA

CD11A I-DOMAIN WITH BOUND MN++

Experimental procedure

Temperature [K]	100
Collection date	1994-09
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	131.130, 45.450, 66.130
Unit cell angles	90.00, 99.80, 90.00

Refinement procedure

Resolution	6.000 - 1.800
R-factor	0.19
Rwork	0.190
R-free	0.25100
RMSD bond length	0.009
RMSD bond angle	24.050 *
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.800
Rmerge	0.040 *
Number of reflections	27543
Completeness [%]	76.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.2	100 *	pH 5.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		5 (mM)
2	1	drop	2-mercaptoethanol	5 (mM)
3	1	drop	protein	15 (mg/ml)
4	1	reservoir	PEG3350	14-20 (%(w/v))
5	1	reservoir		100 (mM)
6	1	reservoir	sodium acetate	50 (mM)